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Hands-on Organ Bath Techniques - Preliminary Detailed Programme
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DAY 1 |
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8.00-8.30 |
Arrival at the gate |
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8.30-8.45 |
Safety instructions at the gate |
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9.00-9.15 |
Welcome coffee & tea |
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9.15-10.15 |
Introduction; Labmagister; staff, trainee; who is who; short programme presentation; safety in the labs-gloves, glasses etc.; first aid |
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10.15-10.30 |
Coffee break |
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10.30-12.30 |
Theoretical background; organ bath; physiological solutions; organ is using for; some important points-temperature-pH-O2/CO2 , types of agonis/antagonist examinations; pA2;pD2 etc. |
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12.30-13.30 |
Lunch break |
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13.30-17.00 |
Introduction to the equipment in the lab; preparation of the physiological solutions; Short presentation of the data management software; calibration; call back old experiments |
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DAY 2 |
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8.00-8.30 |
Arrival at the gate |
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8.30-9.00 |
Welcome coffee & tea |
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9.00-12.30 |
Final dissolutions-bath solution, agonist measurements etc.; execution of the animals-dissection of the organ; mounting organ; spontaneous tone/without spontaneous tone |
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12.30-13.30 |
Lunch break |
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13.30-17.00 |
Agonist&wash out; Concentration-response; ED50 dose+antagonist+ED50 dose |
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Optional social programme at an additional cost |
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DAY 3 |
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8.00-8.30 |
Arrival at the gate |
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8.30-9.00 |
Welcome coffee & tea |
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9.00-12.30 |
Evaluation of the results; data treatment;-calculations-biostatistics; lab-notebook; formal requirements |
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12.30-13.30 |
Lunch break |
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13.30-17.00 |
Opportunity to drill what was learnt; free supervised excercise in the bench/laboratory. |
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DAY 4 |
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8.00-8.30 |
Arrival at the gate |
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8.30-9.00 |
Welcome coffee & tea |
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9.00-12.30 |
Free exercise |
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12.30-13.30 |
Lunch break |
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13.30-17.00 |
Free exercise |
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DAY 5 |
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9.00-17.00 |
Option for one-to-one course for 350€/person |
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Optional social program for the weekend at an additional cost |
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