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DAY 1
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8.00-8.30
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Arrival to the gate
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8.30-8.45
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Safety instructions at the gate
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9.00-9.15
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Welcome caffee&tea
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9.15-10.15
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Introduction; Labmagister; staff ,trainee;who is who; short program presentation;
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10.15-10.30
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Caffee break
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10.30-12.30
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Safety in the labs; sources of risks; self protecting tools; tools and realted risks;
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12.30-13.30
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Lunch break
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13.30-17.00
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Introduction of the of the most frequently used tools and equipment in the lab; pipets; syringes;stirres;heaters; lab balances; centrifuge etc.
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DAY 2
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-10.00
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Pipettes; which for what; how to use them
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10.00-11.00
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Laboratory balances; how to use; which for what and why; spatula
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11.00-12.30
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Dissolution;practicing the use both pipettes and balances
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12.30-13.30
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Lunch break
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13.30-14.30
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Laboratory centrifuge; which for what; safety
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14.30-15.30
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Measuring pH
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15.30-16.30
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Cleaning the lab; finishing the work; personnal hygenie
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DAY 3 - Organ bath assais 1
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-12.30
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Theoretical background; organ bath; physiological solutions; systems; organs are using for;some important points-temperature-pH-O2/CO2 , types of agonis/antagonist examinations; pA2;pD2 etc.
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12.30-13.30
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Lunch break
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13.30-17.00
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Introduction of the equipment in the lab; preparation of the physiological solutions;
Short presentation of the data management software; calibration; call back old experiments
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DAY 4 - Organ bath assais 2
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-10.00
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Final dissolutions-bath solution, agonist measurements etc.; execution of the animals-dissection of the organ; mounting organ;
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10.00- 12.30
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Lunch break
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12.30-13.30
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Agonist&wash out; Concentration-response; ED50 dose+antagonist+ED50 dose; final dissolutions-bath solution, agonist measurements etc.; execution of the animals-dissection of the organ; mounting organ;
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DAY 5 - Organ bath assais 3
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-10.00
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Evaluation of the results; data treatment;-calculations-biostatistics;lab-notebook; formal requirements
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10.00-12.30
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Lunch break
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12.30-13.30
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Opportunity to drill what was learnt; free supervised excercise in the bench/laboratory.
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